Journal: Current Issues in Molecular Biology

Article Title: Hyperthermia Enhances Adeno-Associated Virus Vector Transduction Efficiency in Melanoma Cells

doi: 10.3390/cimb45100537

Figure Lengend Snippet: Expression of AAVR, HSPG1, and HSPG2 receptors for AAV in melanoma cells (A375, G-361, and SK-MEL-1) and normal fibroblasts (CCD-1064Sk). The results are presented as a mean of three independent repeats ± SD. ( A ) Expression levels (2 −dCt ) of receptors for AAV under standard conditions—constitutive levels. Statistically significant differences between CCD-1064Sk and cancer cells are marked with asterisks (**** p < 0.0001). ( B ) Averaged constitutive expressions (2 −dCt ) of receptors for AAV in the examined cells. The expression levels (2 −dCt ) of the three receptors were averaged for each cell line. ( C ) Changes in the expression (2 −ddCt ) of receptors after exposure to hyperthermia. For each receptor, lighter colors indicate expression at 37 °C and darker at 43 °C. Statistically significant differences between 37 °C and 43 °C are marked with asterisks (** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: Cell lines of melanoma A375 (CRL-1619), G-361 (CRL-1424), and SK-MEL-1 (HTB-67) from the Melanoma Cancer Cell Panel (TCP-1013) and the cell line of normal fibroblasts CCD-1064Sk were obtained from the ATCC (American Type Culture Collection, Manassas, VA, USA).

Techniques: Expressing

Journal: Cell adhesion & migration

Article Title: Expression of kindlin-3 in melanoma cells impedes cell migration and metastasis.

doi: 10.1080/19336918.2016.1243645

Figure Lengend Snippet: Figure 6. Analyses of kindlin-3 expression in M10 human melanoma cell line. (A) qRT-PCR analyses of kindlin-1, ¡2 and ¡3 mRNA expression levels in M10 cells and the human chronic myeloid leukemia cell line K562. Values are mean § SD of technical triplicates. A representative experiment from 3 independent experiments is shown. (B) qRT-PCR analyses of kindlin-3 expression in M10, K562 and the human fibroblast cell line 293T. In (A) and (B), GAPDH and actin were used as internal controls. (C) Immunoblot analyses of kindlin-3 protein expression in M10 cells, K562, 293T and the human breast cancer cell line MDA-MB-231 using the anti-kindlin-3 mAb clone 9. Actin serves as loading control. (D) Immunoblot analyses using the anti-kindlin-3 mAb 3D6.

Article Snippet: Human melanoma cell line M10 was purchased from American Type Culture Collection (Manassas, VA) and cultured in RPMI1640 fullmedium containing supplements aforementioned.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Control

Journal: Cell adhesion & migration

Article Title: Expression of kindlin-3 in melanoma cells impedes cell migration and metastasis.

doi: 10.1080/19336918.2016.1243645

Figure Lengend Snippet: Figure 7. Analyses of the migratory properties of M10 cells transfected with GFP-kindlin-3. (A) Flow cytometry analyses and sorting of cells transiently transfected with GFP or GFP-kindlin-3 expression plasmid. (B) Sorted cells were subjected to 2D-random migration assays on fibronectin-coated (2 mg/cm2) coverslip-bottom culture dishes. Twenty cells were tracked and migratory profiles were plotted. Representative data from at least 2 independent experiments are shown. X and Y axes are relabeled with larger fonts for clarity.

Article Snippet: Human melanoma cell line M10 was purchased from American Type Culture Collection (Manassas, VA) and cultured in RPMI1640 fullmedium containing supplements aforementioned.

Techniques: Transfection, Flow Cytometry, Expressing, Plasmid Preparation, Migration

Journal: Life Science Alliance

Article Title: The HDL particle composition determines its antitumor activity in pancreatic cancer

doi: 10.26508/lsa.202101317

Figure Lengend Snippet: WT or Apoa1 KO C57Bl6/J mice were inoculated with 0.5 × 10 6 syngeneic tumor cells either subcutaneously in the right flank (Panc02, B16F10, lung carcinoma cell) or orthotopically in the second mammary fat pad (E0771). (A) Tumor growth kinetics and tumor weight at experimental end stage of the Panc02 pancreatic-cancer model (n = 7/7). (B) Tumor growth kinetics and tumor weight at experimental end stage of the B16F10 melanoma model (n = 13/10 WT and Apoa1 KO mice, respectively). (C) Tumor growth kinetics and tumor weight at experimental end stage of the lung carcinoma cell lung cancer model (n = 9/8). (D) Tumor growth kinetics and tumor weight at experimental end stage of the E0771 breast cancer model (n = 10/13; * P < 0.05; unpaired t test for A, B, C, D).

Article Snippet: The murine melanoma cell line B16F10, Lewis LLCs, and the breast adenocarcinoma cell line E0771 were a kind gift of Prof. Dr. Peter Carmeliet and Prof. Massimiliano Mazzone (VIB Vesalius Research Center, KU Leuven) and maintained in DMEM medium supplemented with 10% FCS, 2 mM L-glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin (complete DMEM).

Techniques: